IRB Barcelona
How to Analyze NGS & Microarray Data with Partek Software
Join us for a complimentary data analysis workshop to learn how to get started with microarray and next generation sequencing (NGS) analysis using Partek software. You will learn to integrate microarray data with NGS data and overcome common analysis challenges.


Microarray
March 28 | 9:30 a.m. - 5:00 p.m.

Agenda
  • Introduction to gene expression analysis
  • Experimental design
  • Statistics

Gene Expression Exercise with Partek® Genomics Suite® and Partek® Pathway™
  • Import, normalization, and quality control
  • Exploratory data analysis - Principle Components Analysis
  • Differential gene expression with the powerful Partek ANOVA implementation
  • Creating lists of significant differentially expression genes
  • Hierarchical clustering and other data visualizations
  • Discovering enriched functional groups using gene ontology
  • Pathway analysis

Optional Self Study
  • Work with your own data
  • Partek trainer available for questions

NGS Analysis
March 29 | 9:30 a.m. - 4:00 p.m.

Agenda
  • Overview of Partek® software for NGS analysis
  • Common tasks in RNA-Seq analysis
 
RNA-Seq Exercise with Partek® Flow®
  • Data import and annotation
  • QA/QC for NGS data
  • Alignment and trimming
  • Quantification
  • Exploratory data analysis - Principal Component Analysis
  • Differential expression analysis - Introduction to Gene Specific Analysis
  • Visualization: dot plots, chromosome viewer, and hierarchical clustering
  • Biological interpretation with gene ontology analysis
  • Save and automate your analysis pipeline
  • Demo on Integration of RNA-Seq and microarray data
 
DNA-Seq Exercise with Partek Flow
  • Alignment
  • Variant calling
  • Variant filtering and prioritization of variants
  • Visualizing variants