MicroRNA Pipeline

The microRNA pipeline trims then aligns microRNAs using Bowtie algorithm customized to take into account short reads. The pipeline also includes pre- and post- alignment QA/QC, as well as a coverage report to measure target effectiveness from an enriched microRNA prep. This pipeline requires a precursor or mature miRBase annotation for the organism of study. Align reads against the reference genome corresponding to the given miRBase annotation. Includes normalization with total count. The microRNA User Guide can be found at http://bit.ly/2iJta3J